Journal:

Article Title: Dentilisin Activity Affects the Organization of the Outer Sheath of Treponema denticola

doi:

Figure Lengend Snippet: Strains and plasmids used in this study

Article Snippet: Pretreatment of T. denticola cells with PMSF just prior to the reaction did not alter either coaggregation reaction. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 T. denticola strain Score a for: P. gingivalis F. nucleatum K1 3 4 ATCC 35405 3 2 K1 + 1 mM PMSF 3 4 ATCC 35405 + 1 mM PMSF 3 2 Open in a separate window a 0, no change in turbidity and no visible coaggregates; 1, finely dispersed coaggregation in a turbid background; 2, definite coaggregation without immediate settling; 3, formation of large settling coaggregates with a slightly turbid supernatant; 4, large coaggregates settling immediately, leaving a water-clear supernatant.

Techniques: Plasmid Preparation, Amplification

Journal:

Article Title: Dentilisin Activity Affects the Organization of the Outer Sheath of Treponema denticola

doi:

Figure Lengend Snippet: Southern blot analysis of T. denticola ATCC 35405 and prtP mutant K1. Chromosomal DNAs from T. denticola ATCC 35405 (lanes 1 and 3) and prtP mutant K1 (lanes 2 and 4) were digested with HindIII and hybridized with a digoxigenin-labeled KpnI-PstI fragment from the prtP gene (lanes 1 and 2) or the KpnI-BamHI fragment from pVA2198 (lanes 3 and 4). Numbers at right are kilobase pairs.

Article Snippet: Pretreatment of T. denticola cells with PMSF just prior to the reaction did not alter either coaggregation reaction. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 T. denticola strain Score a for: P. gingivalis F. nucleatum K1 3 4 ATCC 35405 3 2 K1 + 1 mM PMSF 3 4 ATCC 35405 + 1 mM PMSF 3 2 Open in a separate window a 0, no change in turbidity and no visible coaggregates; 1, finely dispersed coaggregation in a turbid background; 2, definite coaggregation without immediate settling; 3, formation of large settling coaggregates with a slightly turbid supernatant; 4, large coaggregates settling immediately, leaving a water-clear supernatant.

Techniques: Southern Blot, Mutagenesis, Labeling

Journal:

Article Title: Dentilisin Activity Affects the Organization of the Outer Sheath of Treponema denticola

doi:

Figure Lengend Snippet: Gelatin zymography of sonicates of T. denticola ATCC 35405 (lane 1) and K1 (lane 2).

Article Snippet: Pretreatment of T. denticola cells with PMSF just prior to the reaction did not alter either coaggregation reaction. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 T. denticola strain Score a for: P. gingivalis F. nucleatum K1 3 4 ATCC 35405 3 2 K1 + 1 mM PMSF 3 4 ATCC 35405 + 1 mM PMSF 3 2 Open in a separate window a 0, no change in turbidity and no visible coaggregates; 1, finely dispersed coaggregation in a turbid background; 2, definite coaggregation without immediate settling; 3, formation of large settling coaggregates with a slightly turbid supernatant; 4, large coaggregates settling immediately, leaving a water-clear supernatant.

Techniques: Zymography

Journal:

Article Title: Dentilisin Activity Affects the Organization of the Outer Sheath of Treponema denticola

doi:

Figure Lengend Snippet: SDS-PAGE analysis of sonicates of T. denticola ATCC 35405 and K1. Samples from lanes 1 to 4 were treated with PMSF. Lanes 1 and 5, T. denticola ATCC 35405 (without boiling); lanes 2 and 6, T. denticola K1 (without boiling); lanes 3 and 7, T. denticola ATCC 35405 (with boiling); lanes 4 and 8, T. denticola K1 (with boiling). After electrophoresis, the gel was stained with Coomassie brilliant blue R-250. Arrowheads indicate the high-molecular-mass oligomeric protein and the Msp band.

Article Snippet: Pretreatment of T. denticola cells with PMSF just prior to the reaction did not alter either coaggregation reaction. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 T. denticola strain Score a for: P. gingivalis F. nucleatum K1 3 4 ATCC 35405 3 2 K1 + 1 mM PMSF 3 4 ATCC 35405 + 1 mM PMSF 3 2 Open in a separate window a 0, no change in turbidity and no visible coaggregates; 1, finely dispersed coaggregation in a turbid background; 2, definite coaggregation without immediate settling; 3, formation of large settling coaggregates with a slightly turbid supernatant; 4, large coaggregates settling immediately, leaving a water-clear supernatant.

Techniques: SDS Page, Electrophoresis, Staining

Journal:

Article Title: Dentilisin Activity Affects the Organization of the Outer Sheath of Treponema denticola

doi:

Figure Lengend Snippet: Immunoblot analysis of T. denticola ATCC 35405 (lane 1; boiled) and K1 (lane 2; boiled) with antidentilisin serum.

Article Snippet: Pretreatment of T. denticola cells with PMSF just prior to the reaction did not alter either coaggregation reaction. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 T. denticola strain Score a for: P. gingivalis F. nucleatum K1 3 4 ATCC 35405 3 2 K1 + 1 mM PMSF 3 4 ATCC 35405 + 1 mM PMSF 3 2 Open in a separate window a 0, no change in turbidity and no visible coaggregates; 1, finely dispersed coaggregation in a turbid background; 2, definite coaggregation without immediate settling; 3, formation of large settling coaggregates with a slightly turbid supernatant; 4, large coaggregates settling immediately, leaving a water-clear supernatant.

Techniques: Western Blot

Journal:

Article Title: Dentilisin Activity Affects the Organization of the Outer Sheath of Treponema denticola

doi:

Figure Lengend Snippet: Immunoblot analysis of T. denticola ATCC 35405 and K1 with anti-T. denticola ATCC 35405 whole-cell serum. Samples from lanes 1 to 4 were treated with PMSF. Lanes 1 and 5, T. denticola ATCC 35405 (without boiling); lanes 2 and 6, T. denticola K1 (without boiling); lanes 3 and 7, T. denticola ATCC 35405 (with boiling); lanes 4 and 8, T. denticola K1 (with boiling). Arrowheads indicate the high-molecular-mass oligomeric protein and the Msp band.

Article Snippet: Pretreatment of T. denticola cells with PMSF just prior to the reaction did not alter either coaggregation reaction. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 T. denticola strain Score a for: P. gingivalis F. nucleatum K1 3 4 ATCC 35405 3 2 K1 + 1 mM PMSF 3 4 ATCC 35405 + 1 mM PMSF 3 2 Open in a separate window a 0, no change in turbidity and no visible coaggregates; 1, finely dispersed coaggregation in a turbid background; 2, definite coaggregation without immediate settling; 3, formation of large settling coaggregates with a slightly turbid supernatant; 4, large coaggregates settling immediately, leaving a water-clear supernatant.

Techniques: Western Blot

Journal:

Article Title: Dentilisin Activity Affects the Organization of the Outer Sheath of Treponema denticola

doi:

Figure Lengend Snippet: Immunoblot analysis of T. denticola ATCC 35405 and K1 with anti-T. denticola ATCC 35404 Msp serum. Samples from lanes 1 to 4 were treated with PMSF. Lanes 1 and 5, T. denticola ATCC 35405 (without boiling); lanes 2 and 6, T. denticola K1 (without boiling); lanes 3 and 7, T. denticola ATCC 35405 (with boiling); lanes 4 and 8, T. denticola K1 (with boiling). Arrowheads indicate the high-molecular-mass oligomeric protein and the Msp band.

Article Snippet: Pretreatment of T. denticola cells with PMSF just prior to the reaction did not alter either coaggregation reaction. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 T. denticola strain Score a for: P. gingivalis F. nucleatum K1 3 4 ATCC 35405 3 2 K1 + 1 mM PMSF 3 4 ATCC 35405 + 1 mM PMSF 3 2 Open in a separate window a 0, no change in turbidity and no visible coaggregates; 1, finely dispersed coaggregation in a turbid background; 2, definite coaggregation without immediate settling; 3, formation of large settling coaggregates with a slightly turbid supernatant; 4, large coaggregates settling immediately, leaving a water-clear supernatant.

Techniques: Western Blot

Journal:

Article Title: Dentilisin Activity Affects the Organization of the Outer Sheath of Treponema denticola

doi:

Figure Lengend Snippet: Coaggregation activity between T. denticola and P. gingivalis or F. nucleatum

Article Snippet: Pretreatment of T. denticola cells with PMSF just prior to the reaction did not alter either coaggregation reaction. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 T. denticola strain Score a for: P. gingivalis F. nucleatum K1 3 4 ATCC 35405 3 2 K1 + 1 mM PMSF 3 4 ATCC 35405 + 1 mM PMSF 3 2 Open in a separate window a 0, no change in turbidity and no visible coaggregates; 1, finely dispersed coaggregation in a turbid background; 2, definite coaggregation without immediate settling; 3, formation of large settling coaggregates with a slightly turbid supernatant; 4, large coaggregates settling immediately, leaving a water-clear supernatant.

Techniques: Activity Assay

Journal:

Article Title: Dentilisin Activity Affects the Organization of the Outer Sheath of Treponema denticola

doi:

Figure Lengend Snippet: Mean areas of lesions at infection sites after challenge with T. denticola ATCC 35405 and prtP mutant K1. Mice were injected with live T. denticola ATCC 35405 (open bars) or mutant K1 (hatched bars), and lesion areas were determined at the indicated times following infection.

Article Snippet: Pretreatment of T. denticola cells with PMSF just prior to the reaction did not alter either coaggregation reaction. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 T. denticola strain Score a for: P. gingivalis F. nucleatum K1 3 4 ATCC 35405 3 2 K1 + 1 mM PMSF 3 4 ATCC 35405 + 1 mM PMSF 3 2 Open in a separate window a 0, no change in turbidity and no visible coaggregates; 1, finely dispersed coaggregation in a turbid background; 2, definite coaggregation without immediate settling; 3, formation of large settling coaggregates with a slightly turbid supernatant; 4, large coaggregates settling immediately, leaving a water-clear supernatant.

Techniques: Infection, Mutagenesis, Injection